Basic Principles of Biological Electron Microscopy

Authors

Jacob P. Hoogenboom
Department of Imaging Physics, Faculty of Applied Sciences, Delft University of Technology, The Netherlands
https://orcid.org/0000-0003-4539-8772
Arjen J. Jakobi
Department of Bionanoscience, Faculty of Applied Sciences, Delft University of Technology, The Netherlands
https://orcid.org/0000-0002-7761-2027

Keywords:

Electron microscopy, scanning electron microscopy, transmission electron microscopy, cryo-electron microscopy

Synopsis

Biological electron microscopy reveals the molecular machinery of life well beyond the resolution of light microscopy. Foundations of Biological Electron Microscopy is an interactive textbook designed to guide undergraduate and graduate students through how electron microscopy (EM) is used to study biological structures across scales—from individual proteins to entire cells and tissues. Covering all major EM modalities—transmission, scanning, volume, and correlative techniques—this textbook builds a unified framework that connects physical principles of electron microscopy with modern biological applications that illuminate life’s foundational processes at the nanoscale.

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Author Biographies

Jacob P. Hoogenboom, Department of Imaging Physics, Faculty of Applied Sciences, Delft University of Technology, The Netherlands

Jacob Hoogenboom aims to invent, develop, and implement new techniques that cross the boundaries and limitations of existing microscopes in visualizing molecules, molecular functionality, and structural details. Most of his work centres around the combination of light and electron microscopy, physically integrated in a single instrument.

Arjen J. Jakobi, Department of Bionanoscience, Faculty of Applied Sciences, Delft University of Technology, The Netherlands

Arjen J. Jakobi aims to advance cryogenic electron microscopy (cryo-EM) to understand how molecular machines are organised and function in cells. His research focuses on developing new approaches to sample preparation, instrumentation, and computational analysis to expand the range of biological questions that can be addressed using cryo-EM.

Cover image of "Basic Principles of Biological Electron Microscopy"

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Interactive Textbook

Published

August 25, 2026

Categories

Copyright (c) 2026 Jacob P. Hoogenboom, Arjen J. Jakobi

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Details about this monograph

Publication date (01)

2026-08-25

How to Cite

Hoogenboom, J. P., & Jakobi, A. J. (2026). Basic Principles of Biological Electron Microscopy. TU Delft OPEN Books. https://books.open.tudelft.nl/home/catalog/book/270